pmirfp670 n1 (Addgene inc)
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pmirfp670 n1
Pmirfp670 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmirfp670+n1/mIFP-N1+(Plasmid+%2354620)/pm41844235-220-33-34
Average 91 stars, based on 10 article reviews
Pmirfp670 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmirfp670+n1/mIFP-N1+(Plasmid+%2354620)/pm41844235-220-33-34
Average 91 stars, based on 10 article reviews
pmirfp670 n1 - by Bioz Stars,
2026-09
91/100 stars
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Plasmid Preparation:Article Title: Inactive ryanodine receptors sustain lysosomal availability for autophagy by promoting ER-lysosomal contact site formation Article Snippet: .. The Article Title: Spuriously transcribed RNAs from CRISPR-sgRNA expression plasmids scaffold biomolecular condensate formation and hamper accurate genomic imaging Article Snippet: The MS2_EGFP and PP7_mCherry plasmids that encode MCP-EGFP and PCP-mCherry, respectively, were gifts form Dr. Daniel Larson (Addgene plasmids #61764 and #61763) [ ]. .. To generate miRFP670-PSPC1 that encodes PSPC1 (also known as PSP1 α ) fused to miRFP670, the coding region of miRFP670 was first PCR amplified from Article Title: Spuriously transcribed RNAs from CRISPR-sgRNA expression plasmids scaffold biomolecular condensate formation and hamper accurate genomic imaging. Article Snippet: The MS2_EGFP and P7_mCherry plasmids that encode MCP-EGFP and PCPCherry , respectively , were gifts form Dr. Daniel Larson Addgene plasmids #61764 and #61763) [ 20 ]. .. To generate iRFP670-PSPC1 that encodes PSPC1 (also known as PSP1 α) used to miRFP670, the coding region of miRFP670 was first CR amplified from Article Title: Rapid and Robust Generation of Homozygous Fluorescent Reporter Knock-In Cell Pools by CRISPR-Cas9. Article Snippet: To generate sgRNA plasmids for CRISPR/Cas9 genome editing, the doxycyclineinducible sgRNA vector system Fgh1tUT (Addgene, Watertown, MA, USA, Plasmid #70183) was utilised [12]. .. The custom construct Fgh1tUT_miRFP670 was developed in-house by replacing the eGFP gene in Fgh1tUT_GFP with the miRFP670 gene from Article Title: Rapid and Robust Generation of Homozygous Fluorescent Reporter Knock-In Cell Pools by CRISPR-Cas9 Article Snippet: To generate sgRNA plasmids for CRISPR/Cas9 genome editing, the doxycycline-inducible sgRNA vector system Fgh1tUT (Addgene, Watertown, MA, USA, Plasmid #70183) was utilised [ ]. .. The custom construct Fgh1tUT_miRFP670 was developed in-house by replacing the eGFP gene in Fgh1tUT_GFP with the miRFP670 gene from Article Title: Reversible Optical Control of Receptor Tyrosine Kinase Activity and ERK Dynamics Using Azobenzene-Carrying DNA Aptamer Agonist. Article Snippet: Receptor tyrosine kinases (RTKs) play a pivotal role in cell signaling through their activation via dimerization.. Recent studies have demonstrated the importance of the temporal dynamics of RTK activity and downstream signals, such as ERK, in determining the cell fate.. To better understand these dynamics, it is essential to develop methods capable of controlling the RTK activity with high temporal resolution. Polymerase Chain Reaction:Article Title: Spuriously transcribed RNAs from CRISPR-sgRNA expression plasmids scaffold biomolecular condensate formation and hamper accurate genomic imaging Article Snippet: The MS2_EGFP and PP7_mCherry plasmids that encode MCP-EGFP and PCP-mCherry, respectively, were gifts form Dr. Daniel Larson (Addgene plasmids #61764 and #61763) [ ]. .. To generate miRFP670-PSPC1 that encodes PSPC1 (also known as PSP1 α ) fused to miRFP670, the coding region of miRFP670 was first PCR amplified from Amplification:Article Title: Spuriously transcribed RNAs from CRISPR-sgRNA expression plasmids scaffold biomolecular condensate formation and hamper accurate genomic imaging Article Snippet: The MS2_EGFP and PP7_mCherry plasmids that encode MCP-EGFP and PCP-mCherry, respectively, were gifts form Dr. Daniel Larson (Addgene plasmids #61764 and #61763) [ ]. .. To generate miRFP670-PSPC1 that encodes PSPC1 (also known as PSP1 α ) fused to miRFP670, the coding region of miRFP670 was first PCR amplified from Article Title: Spuriously transcribed RNAs from CRISPR-sgRNA expression plasmids scaffold biomolecular condensate formation and hamper accurate genomic imaging. Article Snippet: The MS2_EGFP and P7_mCherry plasmids that encode MCP-EGFP and PCPCherry , respectively , were gifts form Dr. Daniel Larson Addgene plasmids #61764 and #61763) [ 20 ]. .. To generate iRFP670-PSPC1 that encodes PSPC1 (also known as PSP1 α) used to miRFP670, the coding region of miRFP670 was first CR amplified from Construct:Article Title: Rapid and Robust Generation of Homozygous Fluorescent Reporter Knock-In Cell Pools by CRISPR-Cas9. Article Snippet: To generate sgRNA plasmids for CRISPR/Cas9 genome editing, the doxycyclineinducible sgRNA vector system Fgh1tUT (Addgene, Watertown, MA, USA, Plasmid #70183) was utilised [12]. .. The custom construct Fgh1tUT_miRFP670 was developed in-house by replacing the eGFP gene in Fgh1tUT_GFP with the miRFP670 gene from Article Title: Rapid and Robust Generation of Homozygous Fluorescent Reporter Knock-In Cell Pools by CRISPR-Cas9 Article Snippet: To generate sgRNA plasmids for CRISPR/Cas9 genome editing, the doxycycline-inducible sgRNA vector system Fgh1tUT (Addgene, Watertown, MA, USA, Plasmid #70183) was utilised [ ]. .. The custom construct Fgh1tUT_miRFP670 was developed in-house by replacing the eGFP gene in Fgh1tUT_GFP with the miRFP670 gene from other:Article Title: Inactive ryanodine receptors sustain lysosomal availability for autophagy by promoting ER-lysosomal contact site formation. Article Snippet: The split greenFAST probe for detecting ER-lysosomal contact sites was obtained by sequential cloning of gBlocks (from integrated DNA technologies) containing the sequence of C-term splitFASTSEC61 P2A, using NotI and EcoRV, and LAMP1-N-term greenFast, using EcoRV and HindIII in a pcDNA3.1 vector. |